Part:BBa_K3855401:Design

Backbone 3 A-B for Type 2 s assembly in Lactobacillus plantarum

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 1
  Illegal suffix found at 5227
  Illegal SpeI site found at 4907
  Illegal PstI site found at 4941
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal SpeI site found at 4907
  Illegal SpeI site found at 5228
  Illegal PstI site found at 4941
  Illegal PstI site found at 5242
  Illegal NotI site found at 7
  Illegal NotI site found at 5235
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal BamHI site found at 1548
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 1
  Illegal suffix found at 5228
  Illegal SpeI site found at 4907
  Illegal PstI site found at 4941
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 1
  Plasmid lacks a suffix.
  Illegal XbaI site found at 16
  Illegal SpeI site found at 4907
  Illegal SpeI site found at 5228
  Illegal PstI site found at 4941
  Illegal PstI site found at 5242
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 23
  Illegal BsaI.rc site found at 5221
  

Design Notes

Confirm with TypeIIS assembly, codon optimised for Lactobacillus plantarum.

Source

Adapted from the pSIP411 shuttle vector.